I would strongly discourage to use any type of mass spectrometry based quantitation without the use of suitable internal standard (IS), because the use of IS should be an integral part of any MS quantitation including lipidomics. The minimum requirement is the use of at least 1 IS per lipid class to be quantified. More detailed recommendations can be found here:
The second important issue is a manual inspection of your data in addition to software annotation, which is urgently needed to remove false identification and supervise your data. The use of data without supervision cannot be recommended. More details here:
based quantitation without the use of suitable internal standard (IS), because
the use of IS should be an integral part of any MS quantitation including
lipidomics. The minimum requirement is the use of at least 1 IS per
lipid class to be quantified. More detailed recommendations can be found here:
https://lipidomics-standards-initiative.org/guidelines/lipid-species-quantification
The second important issue is a manual inspection of your data
in addition to software annotation, which is urgently needed to remove false
identification and supervise your data. The use of data without supervision
cannot be recommended. More details here:
https://lipidomics-standards-initiative.org/guidelines/lipid-species-identification/general-rules