Lipidomics analysis benefits from extraction with nonpolar organic solvents since polar metabolites, proteins and salts were reduced, leading to a greatly reduced complexity of the samples and MS readout.

  • Procedures need to be evaluated concerning recovery and reproducibility of measured lipids for respective sample matrix
  • Exogenous (it should be verified that they are not present in the sample) internal standards should be added prior to extraction; equilibration with the sample should be evaluated and also verified not present in the sample
  • Frequently applied protocols: