To assure a high data quality it is necessary to validate lipidomic methods - validation should evaluate the following criteria - these data should also be reported:

  • Reproducibility within and from batch to batch
  • Accuracy within and from batch to batch
  • Reproducibility and accuracy for lipid species should be analyzed using the samples subjected to analysis; applicable are either pooled samples or preferred individual samples at different concentration levels.
  • It is not sufficient to evaluate reproducibility and accuracy with standard-mixtures
  • Limit of detection
  • Linear dynamic range
  • Sample carry over
  • Stability of samples including preanalytics