Each batch should include several analytical controls:

  • Blanks to monitor chemical/lipid background and carryover as a sources of artefacts:
    • Solvent blank
    • Internal standard blank
    • Extraction blank
  • Quality control samples (QCs) to demonstrate comparable concentrations and coverage of lipid species between different batches within one or between different laboratories
  • QCs and reference material should have the same matrix as the sample material
  • Reference materials like NIST SRM 1950 are necessary to permit community wide comparability