Positive ion mode

Lipid classes

In-source fragmentation*

Cer

NL of one or two molecules of water - generation of isomeric species like [Cer d34:1+H]+ = [Cer t34:0+H-H2O]+ and isobaric species [Cer d34:1+H]+ = [Cer t34:0+H-H2O]+

CE - FC

NL of FA - generation of cholestadien ion

SM - SPC

NL of FA - generation of SPC

HexCer - Cer

NL of hexose - generation of Cer

SHexCer - HexCer

NL of SO3 - generation of HexCer

Sterols

NL of water - generation of [M+H-H2O]+

DG and MG

NL of water from [M+Na]+ or [M+NH4]+

TG - DG

NL of FA - generation of DG

PS and PC - PA

NL of serine/choline headgroup - generation of PA

LPS and LPC - LPA

NL of serine/choline headgroup - generation of LPA

PL - LPL

NL of FA - generation of LPL

Negative ion mode

Lipid classes

In-source fragmentation*

PL and SL

Generation of [RCOO]-

PS - PA

NL of serine headgroup - generation of PA

LPS - LPA

NL of serine headgroup - generation of LPA

 * In-source fragmentation depends on the type of ion source and ionization conditions like voltages and temperatures